| 质粒名称: | human oct4-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 21153 |
| 存储实验室: | Rudolf Jaenisch |
|
载体骨架基本信息 |
|
| 载体名称: | BluescriptKSII |
| 载体出品公司: | stratagene |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 2381 bp |
| 完整质粒大小: | 122387 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | hOCT4-GFP |
| 物种来源: | 人源 |
| 插入大小: | 120006 bp |
| 基因又名: | hCol1a1 targeting arms |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XhoI(未破坏) |
| 3' 克隆位点: | XbaI (未破坏) |
| 5' 测序引物 | CGATAAGCTTGGTACCGAGC |
| 3' 测序引物 | CCAACACACTATTGCAATGAAA |
|
文献引用方法 |
|
| 材料和方法部分: | human oct4-GFP was a gift from Rudolf Jaenisch (爱迪基因 plasmid # 21153) |
| 参考文献部分: | Nanoparticles for gene transfer to human embryonic stem cell colonies. Green JJ, Zhou BY, Mitalipova MM, Beard C, Langer R, Jaenisch R, Anderson DG. Nano Lett. 2008 Oct . 8(10):3126-30. 10.1021/nl8012665 PubMed 18754690 |
买家导航
human oct4-GFP载体质粒基本信息
human oct4-GFP质粒图谱载体图谱和human oct4-GFP载体序列质粒序列多克隆位点信息
human oct4-GFP质粒载体简介
human oct4-GFP质粒序列载体序列
LOCUS human oct4-GFP 14407 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS human oct4-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14407)
AUTHORS Green JJ, Zhou BY, Mitalipova MM, Beard C, Langer R, Jaenisch R,
Anderson DG
TITLE Nanoparticles for gene transfer to human embryonic stem cell
colonies.
JOURNAL Nano Lett. 2008 Oct . 8(10):3126-30.
PUBMED 18754690
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|human oct4-GFP|
FEATURES Location/Qualifiers
source 1..14407
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 2544..3040
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(2579..2602)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 2973..2992
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 3080..3679
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3080..3099)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 3576..3596
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 4215..4231
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 4216..4232
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
CDS 8307..9023
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(8352..8373)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(8613..8632)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 8960..8981
/label=EGFP-C
/note="EGFP, forward primer"
intron 9055..9627
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind complement(12040..12059)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(12041..12059)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(12146..12163)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(12317..12905)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(12397..12416)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(13076..13936)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 13699..13718
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(13937..14041)
/gene="bla"
/label=AmpR promoter
primer_bind 14109..14127
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(14165..14187)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 14287..14306
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 14387..14405
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 14387..14404
/label=SP6
/note="SP6 promoter, forward primer"
ORIGIN
1 tcgaggtgga caccaccctc aagagcctga gccagcagat cgagaacatc cggagcccag
61 agggcagccg caagaacccc gcccgcacct gccgtgacct caagatgtgc cactctgact
121 ggaagagtgg tgagggcctg ccctagcctc tccctccctc ctactcctgc catgccaggg
181 tccccatgcc catatgtgcc cctaccatat ggtgctggct gctccctttc cctgactcca
241 tcttgccctg ccctaccaca ggagagtact ggattgaccc caaccaaggc tgcaacctgg
301 atgccatcaa agtcttctgc aacatggaga ctggtgagac ctgcgtgtac cccactcagc
361 ccagtgtggc ccagaagaac tggtacatca gcaagaaccc caaggacaag aggcatgtct
421 ggttcggcga gagcatgacc gatggattcc aggtgcgtga gctggacctc agagccagtg
481 ttaggagatg ggctagccca gtgctcagaa gggacatgaa gtcctggagt aggtctctgc
541 taagggtgat ggacagagct gggctgggag gcaggggtct caggtccctg atagtggttc
601 agacacaggc tgccgatggg caggtggtgc ctcctctcga tataacggtg cattgggcag
661 ctctctgagg accctggaca gggaggccag gcaggactag aggttcccgc atagcggctc
721 actcttccct ctctcccctc ccctgcagtt cgagtatggc ggccagggct ccgaccctgc
781 cgatgtggcc atccagctga ccttcctgcg cctgatgtcc accgaggcct cccagaacat
841 cacctaccac tgcaagaaca gcgtggccta catggaccag cagactggca acctcaagaa
901 ggccctgctc ctccagggct ccaacgagat cgagatccgc gccgagggca acagccgctt
961 cacctacagc gtcactgtcg atggctgcac ggtgagtgcc cagaatcccc aggcagggcc
1021 ccacctctcc ggccttgggc tttttggcca ggccatagtg ccctctctcc atcactccca
1081 cgtggtaatg ccccctcccg ttgtctccgc cccaccccag agtcacaccg gagcctgggg
1141 caagacagtg attgaataca aaaccaccaa gacctcccgc ctgcccatca tcgatgtggc
1201 ccccttggac gttggtgccc cagaccagga attcggcttc gacgttggcc ctgtctgctt
1261 cctgtaaact ccctccatcc caacctggct ccctcccacc caaccaactt tccccccaac
1321 ccggaaacag acaagcaacc caaactgaac cccctcaaaa gccaaaaaat gggagacaat
1381 ttcacatgga ctttggaaaa tatttttttc ctttgcattc atctctcaaa cttagttttt
1441 atctttgacc aaccgaacat gaccaaaaac caaaagtgca ttcaacctta ccaaaaaaaa
1501 aaaaaaaaaa agaataaata aataactttt taaaaaagga agcttggtcc acttgcttga
1561 agacccatgc gggggtaagt ccctttctgc ccgttgggct tatgaaaccc caatgctgcc
1621 ctttctgctc ctttctccac accccccttg gggcctcccc tccactcctt cccaaatctg
1681 tctccccaga agacacagga aacaatgtat tgtctgccca gcaatcaaag gcaatgctca
1741 aacacccaag tggcccccac cctcagcccg ctcctgcccg cccagcaccc ccaggccctg
1801 ggggacctgg ggttctcaga ctgccaaaga agccttgcca tctggcgctc ccatggctct
1861 tgcaacatct ccccttcgtt tttgaggggg tcatgccggg ggagccacca gcccctcact
1921 gggttcggag gagagtcagg aagggccacg acaaagcaga aacatcggat ttggggaacg
1981 cgtgtcaatc ccttgtgccg cagggctggg cgggagagac tgttctgttc cttgtgtaac
2041 tgtgttgctg aaagactacc tcgttcttgt cttgatgtgt caccggggca actgcctggg
2101 ggcggggatg ggggcagggt ggaagcggct ccccatttta taccaaaggt gctacatcta
2161 tgtgatgggt ggggtgggga gggaatcact ggtgctatag aaattgagat gcccccccag
2221 gccagcaaat gttccttttt gttcaaagtc tatttttatt ccttgatatt tttctttttt
2281 tttttttttt tttgtggatg gggacttgtg aatttttcta aaggtgctat ttaacatggg
2341 aggagagcgt gtgcggctcc agcccagccc gctgctcact ttccaccctc tctccacctg
2401 cctctggctt ctcaggcctc tgctctccga cctctctcct ctgaaaccct cctccacagc
2461 tgcagcccat cctcccggct ccctcctagt ctgtcctgcg tcctctgtcc ccgggtaccg
2521 ggccccccct cgaccattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
2581 atgcgcttta gcagccccgc tggcacttgg cgctacacaa gtggcctctg gcctcgcaca
2641 cattccacat ccaccggtag cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
2701 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
2761 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
2821 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
2881 gggctcagca gctgggaagg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
2941 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
3001 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcatcc atctagatct
3061 cgagcagctg aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
3121 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
3181 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
3241 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
3301 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
3361 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
3421 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
3481 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
3541 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
3601 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
3661 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
3721 acgaccccat gcatcgatga tatcagatcc ccgggatgca gaaattgatg atctattaaa
3781 caataaagat gtccactaaa atggaagttt ttcctgtcat actttgttaa gaagggtgag
3841 aacagagtac ctacattttg aatggaagga ttggagctac gggggtgggg gtggggtggg
3901 attagataaa tgcctgctct ttactgaagg ctctttacta ttgctttatg ataatgtttc
3961 atagttggat atcataattt aaacaagcaa aaccaaatta agggccagct cattcctccc
4021 actcatgatc tatagatcta tagatctctc gtgggatcat tgtttttctc ttgattccca
4081 ctttgtggtt ctaagtactg tggtttccaa atgtgtcagt ttcatagcct gaagaacgag
4141 atcagcagcc tctgttccac atacacttca ttctcagtat tgttttgcca agttctaatt
4201 ccatcagaag ctggtcgagg tcgacggtat cgataagctt ggtaccgagc tcggatccac
4261 tagtaacggc cgccagtgtg ctggaattcg ccctcagggt acagggaggc ctatgccaac
4321 ttctcattga acaccactcc cagctttcct acccccattt ctcttcacag gtgtgcagag
4381 aagtctacat tcccatgtca agtaaggggg gtgggtagat gggagggcat atgagagcgg
4441 ggcagcaatt ttatgtctga gttggaagga agtttagagt tcattgagtc caaatcctct
4501 ttactaggtg aggaaactga ggcacaggag ggggaggtgc cattagccag ggagtcagag
4561 ccaggactta gaacctggtc catgcctcct agtttgttcc tgaccaatcc tcccagctca
4621 cccactcctt ttgctctcac ctctgactcc ctacaggcta cagctagaga gttgactcct
4681 ctatttgagg taagagtgtc tggatttggg ataaggtgac ctcagtttgc agggctcatg
4741 ggggagatca gagggtcctt tgttgagaag aggggacttg gtccacagtg agcaaaggaa
4801 tttgtctaaa ttgctattaa gggagatcat ctcccaaggt gggccaagcc tgaaccctca
4861 gccctccgtt tcttcccttt cttttgcctt cagattgaca ggcctcggaa gtcaaaataa
4921 gtggtttcct agaccgggtc gagagcaagt ctctattggt cccaactgag ttttttcagc
4981 tggtttttca accaaacagc acctcatctc ccagtgaggg gaagggaagg ctgggctgag
5041 agcagcaagg ctgctcatct cacctctccc cacccagcca tgccagccgc ctcacctggt
5101 ggggagaggt gggcctcacc tgggtcccct ggcagtgctc tgtgaagggt cttgacattg
5161 cactgtaata ataaaggtgt gtgtgaagta tctttttatg gtgactttct aaaacccagg
5221 gaatcatggg accagttctg atgactcagc ctggcttcca gtctcctcca ggcccaacgg
5281 tggcccccag cactggttgg ggcctgggag agctggcctt ggctgaagtg aagccaccta
5341 cccttcaggc ataacaggac agtgagaagg aaggaaagcc tgcctcaacc tcccatcagc
5401 cctgagcacc ccagaagggg gccggctagg agtctaggca tgcaggaggc tgacccctga
5461 ctgggctcat atccagccac aaggcagcca gggatccagg cacccacccc ttgtctgcgt
5521 ccctctcggg aatgggcctc ttgcccaggc cagaaataca ccacctacag tacaaattat
5581 aatctaaaaa caagagggtg gtgttgagtg gggaaattgg ggaaggtgtt ttaggagcca
5641 ctaggaaaat gggcagcagg gactctctgg actggcttgg gaagagcgct tttggggaac
5701 ctggaggatg gcaagctgag aaacactggt gtggagattc cagccaaatc ccaggcctgc
5761 ccctccccct cctctgagag gccgtcttct tggcagacag cagagagatg catgacaaag
5821 gtgccgtgat ggttctgtcc tggggattga gatggctggg gaggggcctc ctcctgttcc
5881 gaagcatgtt cctcccaccc ccaccaggcc ccataatcta cctgcctttt gggcagttaa
5941 aggccgagaa gtgaacacag ctgcaacccc actgccttgt agaccttccg gcagacctgt
6001 ggcaggtatt gaaatgcacg catacaatta ggctcaaaaa gtctacacag acaggagatg
6061 ggcacacgaa cagaggcaac ataagagtgg gggaaaagtc tcaaaagact cacggatgcc
6121 accaagatga agacagctgg ccacgggaca cccatcccct tagaaggcag atagagccac
6181 tgaccccagc agacaagccc aggcagggct gagcctggag cctgcaatga gaagccttac
6241 ttaagtcgac agaggtcagc gtgcccagtc cagacctggc cttctggcct tcgaagctgt
6301 ggggagccct ggcccagagc cccctctgga gcccccagac ttaccccagg ccctccactg
6361 agatcaagtt ttgggagcag acagacaaac atcatccctc acagacaggc attccgttgg
6421 ctattctctt gcaaacagaa tcaagcacta gaccagcagc atgagcctca ggatactcag
6481 gccaggccca gaaaaacaga ccctgaaggg gagcttaggg cagccttcct gcacgcctcc
6541 acaaatcact ctccacctcc tctgcgtctt tctgccagcc agccccacta aacaaagcac
6601 atccctcaat ctgccaggct cggggaggga cgcacgatga agctggacgc ctgagtcccc
6661 cagaggaagg aggaactaga tacctaggtc cctgtggggg gcccttggtg cccgtctgag
6721 gctcagtctt tgaggggatt gcagaggggg gttgctggag ctccttttag cgtctctgaa
6781 ggggattctg tgtgagggga ttgggactgg ggggttgggg agcaggaagc agtccccagg
6841 ggagccatcc aggcccattc aagggttgag cacttgttta gggttagagc tgccccctct
6901 ggggaccggg attgtccagc caaggccatt gtcctgcccc cttcccccag tccctcccag
6961 gcttctttga acctgaagtc agatattttt tctccacacc ccccaccccc tggttttccc
7021 cacccagggc ctagggctgg aggcctgggc cagggaggtg ggggagggag aacggggcct
7081 accgtggtat tagatgtctg agttttggtt gagaggggag caaggaacct gatgtgcagg
7141 ttccatagtg gagggggccc aaagcgggtg tcttatcact ctgtttcagc aaaggttggg
7201 aaactgaggc ccagtcagtc caaagtctgg tcccttgaag gggaagtagg gaccaacccc
7261 ttagtctgtt agatgaggag agtctggagt ctgattctgg aagacggagg ggtgggggga
7321 tgggggggtg gggggatata gcacggaggc cttgtctggc agtctactct tgaagatggg
7381 gtgaaatttg gcaggctggg cagatggtgc caggcaccca ggctgcgggg tggctggatt
7441 tggccagtat cgggatggga atgcctagga ttctggatgg atcgggggaa ggcataaggg
7501 agcagctggc cattgtgctt atggctgttg atgcattgag ggatagcgcc acacacacat
7561 tcaataaatt tgaggagctg agagggtgac tggcccctga aggcacagtg ccagaggtct
7621 gtggagaggg ggtcaagcac ctgggttcct gaagaacatg gaggtgtggg agtgattcca
7681 gacagctggg atgtgcagag cctgagagag tgccagggag cgggttggga gttgaaagtt
7741 gggtgtggtg gctcacgcct ttaatcatga cactgggcgg cagaggcggg aggatttctt
7801 gaggacagga attcaagacc agcctgggta acatagcaag gccccatctc tactaaaaat
7861 aaaaaaacta acagggcaca gtggtccaag cctgtagtcc cagccactta ggaggctgga
7921 gcagaaggat tgctttggcc cagtagatcg aggctacatt gagccatcat tgtactccac
7981 tgcactccag tctgggcaac aaagtgagac cctgtcttaa aaaataaaaa taaaaaaagt
8041 ttctgtgggg gacctgcact gaggtcctgg aggggcgcca gttgtgtctc ccggttttcc
8101 ccttccacag acaccattgc caccaccatt aggcaaacat ccttcgcctc agtttctccc
8161 cccacctccc tctcctccac ccatccaggg ggcggggcca gaggtcaagg ctagtgggtg
8221 ggactgggga gggagagagg ggttgagtag tcccttcgca agccctcatt tcaccaggcc
8281 cccggcttgg ggcgccttcc ttccccatgg tgagcaaggg cgaggagctg ttcaccgggg
8341 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
8401 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
8461 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
8521 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
8581 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
8641 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
8701 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
8761 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
8821 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
8881 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
8941 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
9001 tcggcatgga cgagctgtac aagactagtt attaaggatc ctgagaactt cagggtgagt
9061 ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg
9121 ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc
9181 tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt
9241 ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt
9301 aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact tttttttcaa
9361 ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt
9421 aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt
9481 ggtagaaaca actacatcct ggtcatcatc ctgcctttct ctttatggtt acaatgatat
9541 acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat
9601 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttgt tgtgctgtct
9661 catcattttg gcaaaggatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct
9721 ggtgtggcca atgccctggc tcacaaatac cactgagatc gttttccctc tgccaaaaat
9781 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
9841 ttcattgcaa tagtgtgttg gaatttctcg atcgctagag cggccgccac cgcggtggag
9901 ctcagagaca acttcccaaa gcacaaagca gtttttcccc ctaggggtgg gaggaagcaa
9961 aagactctgt acctattttg tatgtgtata ataatttgag atgtttttaa ttattttgat
10021 tgctggaata aagcatgtgg aaatgaccca aacataatcc gcagtggcct cctaatttcc
10081 ttctttggag ttgggggagg ggtagacatg gggaaggggc tttggggtga tgggcttgcc
10141 ttccattcct gccctttccc tccccactat tctcttctag atccctccat aaccccactc
10201 ccctttctct cacccttctt ataccgcaaa cctttctact tcctctttca ttttctattc
10261 ttgcaatttc cttgcacctt ttccaaatcc tcttctcccc tgcaatacca tacaggcaat
10321 ccacgtgcac aacacacaca cacactcttc acatctgggg ttgtccaaac ctcataccca
10381 ctccccttca agcccatcca ctctccaccc cctggatgcc ctgcacttgg tggcggtggg
10441 atgctcatgg atactgggag ggtgagggga gtggaacccg tgaggaggac ctgggggcct
10501 ctccttgaac tgacatgaag ggtcatctgg cctctgctcc cttctcaccc acgctgacct
10561 cctgccgaag gagcaacgca acaggagagg ggtctgctga gcctggcgag ggtctgggag
10621 ggaccaggag gaaggcgtgc tccctgctcg ctgtcctggc cctgggggag tgagggagac
10681 agacacctgg gagagctgtg gggaaggcac tcgcaccgtg ctcttgggaa ggaaggagac
10741 ctggccctgc tcaccacgga ctgggtgcct cgacctcctg aatccccaga acacaacccc
10801 cctgggctgg ggtggtctgg ggaaccatcg tgcccccgcc tcccgcctac tcctttttaa
10861 gctttttctt tctttctttc ttttttttct taaattcttg cttcctcttc ccccctcttc
10921 tccgtccctt ctctttccct tcaccttctg cctgctcctt cccagaggcc ctgagaaggc
10981 gaggcccgga gggaaggtga ctgttcggct cccccatctc aggtaagggg gacagcgagt
11041 gtggtcagcg ggggacagag aatccagggc gtcagaagtc cagagagaac caggcacccg
11101 gagccaggga gacagcgaga cgacagctgg tcacctctct cacctcatct caggtctaaa
11161 cccaaaaaga acaaaaagcc cgcactcctc cctaaggtct ccagagagat ggacaagtct
11221 gtagatacca tccatcagca atactaatat cgtgatgtca ttaagtattc atggccgttc
11281 attatggcct tcaataattc atcattagca cttctaccac acttaggaca gtgtagaaag
11341 ggaatcagtg tagagaggat aagaatgatg gcatataaat gaaggcttat tcatatgcaa
11401 attacccaaa gttgtgtgat gcctatccat ctctctttct ctcttttttt tgagacagag
11461 tctcactctg tcacccaggc tggagtgcgg tggcgcagtc tcagctcact gcaacctccg
11521 cctcccaggt tcaagcaatt ctcatgcctc agcctcccaa gtaactggga tgacagccat
11581 gcactaccac gcccagctaa tttttatatt tttagtagag tcagggtttt accatgttag
11641 ccagcctggt ctcgaactcc tggcctcaag tgatccacct gcctcagcct ctcaaagtgc
11701 tgggattgcg gacatgagcc acagcaccag gctccatctc ttgatcaatg tgttgattgc
11761 ttggtgatgc ctgccatgct gcccaggaaa ccctgtccac ttggacctct gggactttag
11821 gaccctggct gtccaggcca catccccatc tgccctcgtg ggggacccag ggacatcctg
11881 agcctggcat ccaaggctct acctggccac tacccacctg cccaggctca ctgtcccctc
11941 ccctgcccac atctgtcaca ccaaccaaaa agggtggcca acagttctca caaatgcact
12001 gcatttcccc acttccaggc cttctagatc tgccggtctc cctatagtga gtcgtattaa
12061 tttcgataag ccaggttaac ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
12121 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
12181 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
12241 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
12301 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
12361 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
12421 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
12481 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
12541 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
12601 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
12661 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
12721 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
12781 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
12841 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
12901 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
12961 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
13021 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
13081 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
13141 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
13201 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
13261 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
13321 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
13381 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
13441 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
13501 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
13561 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
13621 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
13681 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
13741 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
13801 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
13861 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
13921 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
13981 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
14041 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
14101 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
14161 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
14221 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
14281 actatgcggc atcagagcag attgtactga gagtgcacca tatggacata ttgtcgttag
14341 aacgcggcta caattaatac ataaccttat gtatcataca catacgattt aggtgacact
14401 atagaac
//
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human oct4-GFP质粒载体应用举例
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